The smart Trick of flow cytometer That Nobody is Discussing
The smart Trick of flow cytometer That Nobody is Discussing
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Facts-enriched characterization of mobile populations. Morphological information and facts from pictures can add for the richness of flow cytometry information. For instance, the determine displays an in any other case conventional apoptosis assay applying Annexin V and PI, introducing cell imaging to characterize cells in Each and every population to reveal morphologically distinct characteristics. These insights couldn't have been received from multiplex staining by yourself.
Regardless of whether you’re a starter or an authority, explore our assets to advance your investigation and know-how in flow cytometry.
Incorporate acoustic focusing technologies with superior-parameter Examination for versatile spectral unmixing and conventional workflows.
The award-successful Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating with a higher-pace brightfield digital camera for simultaneous substantial throughput flow cytometry and significant resolution brightfield imaging.
"An image isn't really fairly truly worth 1000 dots, nevertheless it seriously helps to see photos. It is great to see photos that correlate with staining. By way of example, AnnexinV-optimistic cells which can be morphologically unique than healthy cells, or CD14+ monocytes which are larger sized and more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a qualified consumer has gated singlets confidently. After analyzing the manual singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate consists of more than four% aggregates.
Optimizing gating system. Even strong manual singlet gating is error-prone and continues to be a subjective selection point in Just about all flow cytometry assays. Imaging can be used to confirm and change gates to incorporate only solitary cells of interest.
Regular impression quality even at substantial throughput Acoustic focusing and also a significant-speed camera Merge to image these T cells continuously at reduced or substantial flow costs. Very easily modify emphasis and camera options to satisfy experimental specifications.
The Attune NxT cytometer is definitely the reliable workhorse instrument your lab can count on for reliable check here and trusted overall performance. The Attune CytPix product features a superior-speed brightfield digicam and AI-pushed graphic Evaluation software package, with the latest updates which includes equipment Studying applications and an intuitive user interface, enabling Increased information precision, accuracy, and quicker insights into sample biology.
Buyers can see occasion images in real-time to verify sample excellent, enhance protocols, and record the morphology of every mobile populace recognized for Examination. Imaging capture also Added benefits from acoustic focusing, which helps you to place the cells in order that a sharp, centered image is obtained.
AI-pushed automatic impression Evaluation With all the Attune Cytometric Software package translates occasion functions into unique morphology parameters that could be coupled with regular fluorescence and scatter parameters to expose really reproducible inhabitants changes and one of a kind subsets.
We Earlier demonstrated the strength of imaging Motor vehicle-T/Ramos cell interactions. Allow’s take a look at just the inhabitants of finest interest, the double beneficial activities, to learn more. We could now use prolonged morphological parameters (here, circularity and skewness of depth) to further take a look at the options of those populations and refine gating on these activities, raising data robustness.
Leverage the power of Highly developed engineering and innovation to accelerate your discoveries. Have confidence in from the Attune Flow Cytometer family to provide the reliability, velocity, and precision that set your operate aside.
Flexible payment prepare from Agilent Economic Solutions let you have the important instrumentation you would like for immediate task, or to mature your lab.
Gating on these prolonged parameters lets you speedily and accurately determine populations of curiosity and confirm gating method with little or no guide overview. The Attune computer software delivers access to point out-of-the-artwork equipment Finding out Examination resources in an expertly made person interface, rendering it exceptionally simple to operate.
The Attune Xenith cytometer is often a superior-parameter spectral analyzer, giving both of those spectral unmixing and conventional flow capabilities for comprehensive and flexible mobile analysis.
Agilent Worth Promise guarantees you a minimum of ten years of instrument use within the day you purchase. Usually, we will credit you with the residual worth of the procedure toward an upgraded product.
What gave the impression to be a homogeneous population is actually a lot more varied—an insight that should be deemed when interpreting final results.